| Name | 5a-Pregnane-3,20-dione |
| Description | 5a-Pregnane-3,20-dione is a biologically active 5-alpha-reduced metabolite of plasma progesterone. |
| Cell Research | MCF-7 cells were grown without or with 5a-Pregnane-3,20-dione (10(-9)-10(-5) M), and the effects on cell and nuclear morphology, adhesion plaques, vinculin and actin expression, actin polymerization, and microfilament distribution were examined by immunohistochemistry, morphometry (using confocal microscopy and digital computer imaging analysis), and Western blotting.?Treatment of cells with 10(-9)-10(-6) M 5a-Pregnane-3,20-dione resulted in dose-dependent decreases in cell area, cell-to-cell contacts, and attachment to the substratum, and increases in variation in nuclear area.?These changes in the 5alphaP-treated cells were accompanied by decreases in vinculin-containing adhesion plaques, vinculin expression, polymerized actin stress fibers, and decreases in insoluble and increases in soluble actin fractions. |
| In vitro | the endogenous progesterone metabolite,5a-Pregnane-3,20-dione, may be involved in promoting breast neoplasia and metastasis by affecting adhesion and cytoskeletal molecules. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 5 mg/mL (15.80 mM), Sonication and heating are recommended.
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| Keywords | Inhibitor | inhibit | 5a-Pregnane-3,20-dione | 5aPregnane3,20dione | Endogenous Metabolite | 5a Pregnane 3,20 dione |
| Inhibitors Related | Potassium gluconate | 5-Fluorouracil | Myricetin | Acetaminophen | Fumaric acid | NSC 42196 | Betaine | Glucosamine | Formamide | Oleamide | Glycerol | Oleic acid |
| Related Compound Libraries | Anti-Tumor Natural Product Library | Bioactive Compound Library | Natural Product Library | NO PAINS Compound Library | Natural Product Library for HTS | RO5 Drug-like Natural Product Library | Human Endogenous Metabolite Library | Human Endogenous Metabolite Compound Library Plus | Bioactive Compounds Library Max | Human Metabolite Library |
United States
