1. Product information
| Product Name: | 5,5′-Dithiobis(2-nitrobenzoic acid) |
| Synonyms: | DTNB;ellmann's reagent;ELLMAN'S REAGENT;ELLMANS' REAGENT;BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE;3-CARBOXY-4-NITROPHENYL DISULFIDE;Ellmsn'sreagent;Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans |
| CAS: | 69-78-3 |
| MF: | C14H8N2O8S2 |
| MW: | 396.35 |
| EINECS: | 200-714-4 |
| Product Categories: | marker;Phenyls & Phenyl-Het;Phenyls & Phenyl-Het |
| Mol File: | 69-78-3.mol |
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| 5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties |
| Melting point | 240-245 °C (dec.)(lit.) |
| Boiling point | 671.9±55.0 °C(Predicted) |
| density | 1.67 |
| refractive index | 1.6900 (estimate) |
| storage temp. | Keep in dark place,Sealed in dry,Room Temperature |
| solubility | Do you have solubility information on this product that you would like to share |
| form | Yellow solid |
| pka | 1.57±0.25(Predicted) |
| color | Pale Yellow to Light Yellow |
| Water Solubility | Soluble in water and ethanol. |
| 5,5′-Dithiobis(2-nitrobenzoic acid) Usage And Synthesis |
| Chemical Properties | Crystalline |
| Uses | 5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample. |
| Uses | Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content. |
| Uses | 5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups. |
| Definition | ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups. |
| General Description | 5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3. |
2. Packaging
For powders: normal is 25kgs/Drum or bag, or larger/smaller package as request.
For liquids: normal 25kgs/drum, 180-300kgs/bucket, or IBC, determined by the nature of the product.
Or smaller package 1kg/bottle, 10kgs/bottle as request.
3. Shipping
4. Contact information
For more details, pls contact us freely.
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