Basic information Safety Related Supplier

ChemicalBook Product Catalog Biochemical Engineering Saccharides Disaccharides 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside

1/1

5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside NEW

$31.00 10mg

$54.00 25mg

$81.00 50mg

Min. Order
Purity99.80%
Cas No177966-52-8
Supply Ability10g
Update time2025-09-11

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

VIP6Y

United States

United States

show more

Enterprise Verified

Business Bank account Basic Contact Infomation Business Address Trade Company

Chemical Properties

Product Name	5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside
CAS No	177966-52-8
EC-No
Min. Order
Purity	99.80%
Supply Ability	10g
Release date	2025/09/11

Product Introduction

Bioactivity

Name	5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside
Description	5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside is a chromogenic compound used to detect cellobiohydrolases.
In vitro	I. Cellulase activity detection 1. Prepare substrate solution: Dissolve 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside in an appropriate buffer solution, usually at a concentration between 0.5-1 mM. Commonly used buffer solutions include citric acid-phosphate buffer (pH 5.0-7.0) or Tris-HCl buffer (pH 7.5-8.5). 2. Add sample: Add the sample to be tested (e.g., bacterial culture or extract containing cellulase) to the substrate solution. The sample may contain related enzymes such as β-glucosidase. 3. Reaction conditions: Incubate the reaction system at a suitable temperature (e.g., 37°C) for a reaction time of 30 minutes to 2 hours. The enzyme-catalyzed reaction produces a blue product. 4. Stop the reaction: Stop the reaction by adding an appropriate chemical reagent (e.g., an acidic buffer) to prevent further activity of the enzyme. 5. Observe and analyze: Observe the blue product in the sample by naked eye or microscope. The appearance of blue products indicates the activity of cellulase. The activity of the enzyme can be evaluated by quantitatively analyzing the intensity of blue staining. 2. Fiber biotransformation research 1. Cultivation of microorganisms: Cultivate fiber-degrading microorganisms, such as cellulose-degrading fungi or bacteria, in appropriate culture media. 2. Addition of substrate: Add 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside solution. 3. Reaction observation: Monitor the progress of the degradation reaction by color change. The color of the reaction product can help determine whether the microorganism has the ability to degrade cellulose. 3. High-throughput screening 1. Sample preparation: Process a large number of microbial samples (such as different strains or genetically engineered strains) in batches. 2. Substrate reaction: Mix 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside solution with the sample. 3. Color detection: Use automated equipment to detect the blue reaction in different samples and quickly screen out efficient cellulose-degrading strains or enzymes. IV. Enzyme activity determination 1. Prepare substrate solution: dissolve 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside in buffer. 2. Add test enzyme solution: add solution containing test cellulose hydrolase or other related enzymes. 3. Observe reaction results: evaluate enzyme activity based on color change.
Storage	store at low temperature,keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Solubility Information	DMSO : 22.5 mg/mL (39.42 mM), Sonication is recommended.
Keywords	5-Bromo-4-chloro-3-indoxyl-β-D-cellobioside \| 5-Bromo-4-chloro-3-indoxyl-beta-D-cellobioside \| 5Bromo4chloro3indoxylbetaDcellobioside \| 5-Bromo-4-chloro-3-indoxyl-b-D-cellobioside \| 5 Bromo 4 chloro 3 indoxyl beta D cellobioside

show more

Company Profile Introduction

You may like

Recommended supplier

MSDS|CAS|CAS DataBase|Pricacy|Terms|About Us|New Products| Contact us|Computer Version
Chemical Book