| Name | 3BDO |
| Description | 3BDO is a new mTOR activator. 3BDO inhibits autophagy. |
| In vitro | Phosphorylation of RPS6KB1 and EIF4EBP1 is significantly increased by 3BDO with vector alone but suppressed with FKBP1A overexpression. Rapamycin fails to decrease the phosphorylation of MTOR and RPS6KB1 in the presence of 3BDO. 3BDO suppresses the increase in MAP1LC3B puncta induced by rapamycin and inhibits its effect in HUVECs. Phosphorylation of Ser residues is decreased in HUVECs treated with 10 μM rapamycin, and 60 μM 3BDO reverses this phosphorylation. These results demonstrate that 3BDO suppresses the increased MAP1LC3B puncta, MAP1LC3B-II levels, and decreased SQSTM1 protein levels induced by rapamycin. Additionally, 3BDO decreases FLJ11812 levels in HUVECs in a dose- and time-dependent manner, while overexpression of FLJ11812 reverses the inhibition of autophagy induced by 3BDO[1]. |
| In vivo | Immunofluorescence assay demonstrates that treatment with 3BDO enhances p-p70S6K levels while reducing ATG13 protein levels in the plaque endothelium of mice, without affecting the phosphorylation of mTOR's immediate downstream targets p70S6K and 4EBP1. Comparatively, in apoE-/- mice, 3BDO treatment notably inhibits endothelium autophagy and apoptosis, thereby offering protection against endothelial injury in atherosclerosis. Furthermore, 3BDO treatment contributes to the stabilization of established atherosclerotic lesions in apoE-/- mice and results in a significant reduction in the serum levels of IL-6 and IL-8. |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO : 150 mg/mL (458.25 mM)
Ethanol : 20 mg/mL (61.10 mM), Sonication is recommended.
|
| Keywords | Autophagy | mTOR | Apoptosis | Mammalian target of Rapamycin | Inhibitor | 3BDO | inhibit |
| Inhibitors Related | Stavudine | 5-Fluorouracil | Sodium 4-phenylbutyrate | L-Ascorbic acid | Hydroxychloroquine | Guanidine hydrochloride | Taurine | Tributyrin | Curcumin | Paeonol | Naringin | Gefitinib |
| Related Compound Libraries | Anti-Lung Cancer Compound Library | Glycolysis Compound Library | Anti-Pancreatic Cancer Compound Library | Bioactive Compound Library | Kinase Inhibitor Library | Anti-Breast Cancer Compound Library | Anti-Cancer Metabolism Compound Library | Neuroprotective Compound Library | Anti-Aging Compound Library | Bioactive Compounds Library Max |
United States
