| Name | 2,3-Dehydro-2-Deoxy-N-Acetylneuraminic Acid |
| Description | 2,3-Dehydro-2-Deoxy-N-Acetylneuraminic Acid is a pan-sialidase (neuraminidase, NEU) inhibitor with inhibitory activity against sialidases 1–4, with an IC50 of 43–143 μM, and also exhibits inhibitory activity against various influenza viruses. |
| In vitro | Methods:
To evaluate the effect on sialidase activity in pancreatic β-cells, INS-1D cells were used, and in vitro enzymatic activity was measured using the substrate 4MU-Neu5Ac (2,3-Dehydro-2-Deoxy-N-Acetylneuraminic Acid).
Results:
N-acetyl-2,3-dehydro-2-deoxyneuraminic acid (DANA / Neu5Ac2en) significantly inhibited sialidase activity in INS-1D cells at concentrations ranging from 10 to 100 μM in a dose-dependent manner [1]. |
| In vivo | Methods:
A mouse model of pulmonary fibrosis was established using bleomycin. Starting on day 10 after bleomycin administration, mice were intraperitoneally injected with 2,3-Dehydro-2-Deoxy-N-Acetylneuraminic Acid (DANA) at a dose of 10 mg/kg daily. Treatment continued until day 21, at which point the animals were euthanized for tissue analysis.
Results:
Inhibition of sialidase activity starting on day 10 after bleomycin administration significantly attenuated pulmonary fibrosis, suggesting that DANA exerts anti-fibrotic effects.[1] |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature. |
| Solubility Information | DMF : < 1 mg/ml (insoluble), Sonication is recommended.
DMSO : 1 mg/mL (3.43 mM), Sonication is recommended.
PBS (pH 7.2) : 10 mg/mL (34.33 mM), Sonication is recommended.
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| Keywords | 2,3-Dehydro-2-deoxy-N-acetylneuraminic acid | 2,3Dehydro2deoxyNacetylneuraminic acid | 2,3 Dehydro 2 deoxy N acetylneuraminic acid |
| Inhibitors Related | Acetylcysteine | α-Vitamin E | Molnupiravir | AEBSF hydrochloride | Umifenovir hydrochloride | Nitazoxanide | Camphor | Curcumin | Naringenin | Salcomine | Coumarin | Crystal Violet |
United States
