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ChemicalBook Product Catalog 2-NBDG

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2-NBDG NEW

$37.00 1mg

$37.00 1mg

$97.00 5mg

$97.00 5mg

$155.00 10mg

$155.00 10mg

Min. Order
Purity≥98%
Cas No186689-07-6
Supply Ability10g
Update time2024-11-19

TargetMol Chemicals Inc.

TargetMol Chemicals Inc.

5YR

United States

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Chemical Properties

Product Name	2-NBDG
CAS No	186689-07-6
EC-No
Min. Order
Purity	≥98%
Supply Ability	10g
Release date	2024/11/19

Product Introduction

Bioactivity

Name	2-NBDG
Description	2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability.
In vitro	METHODS: Flow cytometry was used to detect glucose uptake: 1, Cells were seeded at 1*104/well in 96-well plates, and the experiment was performed within 24-48 h. The cells were incubated at 37 ℃ for 10-180 min. 2, Remove the cell culture medium, add fresh medium containing 2-NBDG (5-40 µM), and incubate for 10-180 min at 37 ℃. 3. Remove the medium and wash twice with pre-cooled PBS to stop the 2-NBDG uptake reaction. Resuspend in fresh medium and perform flow cytometry within 30 min. [1]
In vivo	METHODS: Glucose uptake by circulating breast cancer cells was detected by fluorescent microscopy: 1. A mouse blood sample (100 µL/mouse) was collected by puncturing the mouse saphenous vein. 2. Incubate the blood sample containing circulating breast cancer cells with 2-NBDG (5 µg/100 µL blood) for 30 min at 37℃ in a dark incubator. 3. Add the magnetic bead suspension (1µL 1%) to 100 µL of blood sample and incubate for 30 min at 4°C with gentle shaking to promote binding of the magnetic beads to the circulating breast cancer cells. 4. Separate the circulating breast cancer cells from the blood using a magnetic separation rack, wash with PBS 3 times, resuspend in 100 µL PBS and transfer to a 96-well cell plate. 5. 2-NBDG uptake by circulating breast cancer cells was examined under a fluorescence microscope equipped with a 488 nm filter. Large cells with a fluorescent signal derived from fluorescent 2-NBDG uptake by cells were counted as hypermetabolic circulating breast cancer cells, and small-sized normal mouse blood cells (lymphocytes and RBCs) showed no or little 2-NBDG fluorescent signal. [2]
Storage	store at low temperature,keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	H2O : 5 mg/mL (14.61 mM), Sonication and heating to 60℃ are recommended.
Keywords	2 NBDG \| 2-NBDG \| Inhibitor \| inhibit \| 2NBDG

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Company Profile Introduction

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