4H-1,4-Benzoxazine-4-carboxylic acid, 2,3-dihydro-, 7-[(4-chlorophenyl)methyl]hexahydro-1,3-dioxoimidazo[1,5-a]pyrazin-2-yl ester

ABC99 is a Selective Irreversible Inhibitors of the Wnt-Deacylating Enzyme, ABC99 inhibited NOTUM with an IC50 value of 13 nM and showed excellent selectivity across the serine hydrolase family, as initially assessed by gel-based ABPP of soluble and membrane proteomes from SW620 cells. ABC99 preserves Wnt-mediated cell signaling in the presence of NOTUM and was also converted into an ABPP probe for visualizing NOTUM in native biological systems.