J14 (0-100 μM; 0-96 hours; A549 cells) treatment inhibits the growth of A549 cells in a concentration- and a time-dependent manner, and its half inhibitory concentration for the growth of A549 cells was 15.7 μM.
J14 (20 μM; 48-72 hours; A549 cells) treatment causes not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9. J14 induces oxidative damage to mitochondria, resulting in caspase-mediated apoptosis.
J14 treatment significantly increases the accumulation of sulfinic peroxiredoxins and intracellular ROS. Excess accumulation of intracellular ROS causes oxidative damage, leading to cell death. J14 significantly induces cell death in A549 cells in a time-dependent manner, resulting in approximately 40% cell death in 96 hours.
J14 induces oxidative mitochondrial damage and apoptosis.
Cell Viability Assay
| Cell Line: | A549 cells |
| Concentration: | 0-100 μM |
| Incubation Time: < /td> | 0 hour, 24 hours, 48 hours, 72 hours, 96 hours |
| Result: | Inhibited the growth of A549 cells in a concentration- and a time- dependent manner. |
Western Blot Analysis
| Cell Line: | A549 cells |
| Concentration: | 20 μM | < /tr>
| Incubation Time: | 48 hours, 72 hours |
| Result: | Caused not only the release of cytochrome c into the cytosol, but also the activation of caspase -3 and caspase-9. |
