800CW NHS ester (IRDye 800CW NHS equivalent) is an amine reactive near-infrared high water-soluble fluorescent dye, it can be labeled to biomolecules directly, such as primary antibody, secondary antibody, peptide, etc.
The conjugates can be used as a probe for Western blot, flow cytometry, immunocytochemical assays, including In-Cell Western assays and On-Cell Western cell-based assays, protein arrays, microscopy, tissue section imaging, in vivo imaging and optical probe development.
800CW dye-conjugated agents and probes are currently propelling more than a dozen Phase I or Phase II clinical trials, more than any other near-infrared fluorescent dye on the market. IRDye a registered trademark of LI-COR.
[1] Kovar J, et al. Near infrared probes for biochemical, cellular, and whole animal analysis of disease processes. Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications. SPIE, 2009, 7190: 71-78.
[2] Reeing F, et al. A Facile and Reproducible Synthesis of Near-Infrared Fluorescent Conjugates with Small Targeting Molecules for Microbial Infection Imaging. ACS Omega. 2020 Aug 26;5(35):22071-22080. DOI:
10.1021/acsomega.0c02094
