INDIGO’s Mouse Glucocorticoid Receptor (nr3c1) nuclear receptor assay utilizes proprietary non-human cells engineered to provide constitutive high-level expression of full-length, unmodified Mouse Glucocorticoid Receptor (nr3c1), a ligand-dependent transcription factor, commonly referred to as GR. INDIGO’s Reporter Cells include the luciferase reporter gene functionally linked to a GR-responsive promoter. Thus, quantifying changes in luciferase expression in the treated reporter cells provides a sensitive surrogate measure of the changes in GR activity. Luciferase gene expression occurs after ligand-bound GR undergoes nuclear translocation. DNA binding, recruitment and assembly of the co-activators and accessory factors required to form a functional transcription complex, culminating in expression of the reporter gene. Unlike some other cell-based assay strategies, the readout from INDIGO’s reporter cells demands the same orchestration of all intracellular molecular interactions and events that can be expected to occur in vivo. mGR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments. [INDIGO Catalog No. M00201, M00201-32]WARNING This product is not for human or veterinary use.
