5-chloro-4-[(2Z)-2-[(3-hydroxyphenyl)methylidene]hydrazinyl]-1H-pyridazin-6-one

L82-G17 is a selective uncompetitive inhibitor of DNA ligase I (LigI).

3-Hydroxy-benzaldehyde (5-Chloro-6-oxo-1,6-dihydro-pyridazin-4-yl)-hydrazone can be used to determine the structure-activity relationships among DNA ligase inhibitors.

Among the LigI-selective inhibitors, L82-G17 exhibited increased activity against and selectivity for LigI compared with L82. Notably. L82-G17 is an uncompetitive inhibitor of the third step of the ligation reaction, phosphodiester bond formation. Cells expressing LigI were more sensitive to L82-G17 than isogenic LIG1 null cells._x000D_ _x000D_ Reference: DNA Repair (Amst). 2017 Dec;60:29-39. https://pubmed.ncbi.nlm.nih.gov/29078112/

L82-G17 is an uncompetitive DNA ligase I (Lig I)-selective inhibitor.