Cayman’s Glutamine Fluorometric/Colorimetric Assay Kit provides both fluorometric and colorimetric methods for measuring glutamine in biological samples such as cell culture supernatants, cell lysates, tissue homogenates, serum, and plasma. In the assay, the Glutamine Converter Enzyme catalyzes the conversion of glutamine to glutamate, which is further converted to α-ketoglutarate (α-KG) and hydrogen peroxide (H2O2) in the presence of the Glutamine/Glutamate Developer Enzyme Mix. MaxiProbe reacts with H2O2, forming a compound that can be easily quantified at excitation and emission wavelengths of 535±5 and 590±10 nm, respectively. Alternatively, the absorbance of the product can be measured at 570 nm. Background from glutamate and H2O2 in the sample is determined by omitting the converter enzyme in the sample background reactions. This assay offers the option of measuring fluorescence or absorbance. It is at the user’s discretion to choose the mode of measurement (and corresponding standard curve preparation) that best fits their needs. When read fluorometrically, this assay has a range of 0-40 μM and a lower limit of detection (LLOD) of 1.6 μM. When read colorimetrically, the range is 0-400 μM with an LLOD of 2.3 μM.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
