A bright, red-fluorescent probe used for detection TCO-tagged biopolymers. AZDye 568 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must AZDye 568 Tetrazine probe would of great value.Tetrazines demonstrate exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In addition, inverse-Electron-Demand Diels-Alder reaction of tetrazines trans-cyclooctenea forms a stable covalent bond and does not require Cu-catalyst or elevated temperatures.AZDye 568™ is a bright, and highly photostable, orange-fluorescent probe optimally excited by the 568 nm laser line on the Ar-Kr mixed-gas laser. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of low-abundance targets.
