DL-Glyceraldehyde-3-phosphate (GADP) is an intermediate in several metabolic pathways, including glycolysis and gluconeogenesis. In glycolysis, it can be derived from fructose 1,6-bisphosphate by fructose bisphosphate aldolase in a reaction that also produces dihydroxyacetone phosphate (DHAP). Triose phosphate isomerase reversibly converts DHAP to GADP. GADP is processed to 1,3-bisphosphoglycerate by glyceraldehyde phosphate dehydrogenase and NAD+.
The level of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) activity in HEK 293 cells was aβeβed by measuring the reduction of NAD+ by DL-Glyceraldehyde 3-phosphate.
ChEBI: Glyceraldehyde 3-phosphate is an aldotriose phosphate that is the 3-phospho derivative of glyceraldehyde. It is an important metabolic intermediate in several central metabolic pathways in all organisms. It has a role as a human metabolite, a plant metabolite and an Escherichia coli metabolite. It is an aldotriose phosphate and an aldehyde. It is functionally related to a glyceraldehyde. It is a conjugate acid of a glyceraldehyde 3-phosphate(2-).
Glyceraldehyde 3-phosphate is an intermediate in several metabolic pathways, including glycolysis and gluconeogenesis. It is bactericidal and a potent inhibitor of growth in E. coli.
In vivo, phosphatidylethanolamine and phosphatidylglycerol accumulation are inhibited to the same extent by the addition of DL-Glyceraldehyde 3-phosphate to a culture of E. coli[2].
