NF 157
NF 157 性质
| 储存条件 | Desiccate at RT |
|---|---|
| 溶解度 | 可溶于DMSO（少许）、甲醇（少许） |
| 形态 | 粉末 |
| 颜色 | 米白色至粉红色 |
| 水溶解性 | Soluble to 50 mM in water |
| 稳定性 | 吸湿性 |
NF 157 用途与合成方法
pKi: 7.35 (P2Y11)
IC50: 463 nM (P2Y11), 1811 µM (P2Y1), 170 µM (P2Y2)
Ki: 44.3 nM (P2Y11), 187 µM (P2Y1), 28.9 µM (P2Y2)
NF157 displays selectivity for P2Y11 over P2Y1 (>650-fold), P2Y2 (>650-fold), P2X2 (3-fold), P2X3 (8-fold), P2X4 (>22-fold), and P2X7 (>67-fold) but no selectivity over P2X1.
NF157 (30 and 60 µM; 24 hours) causes a significant reduction in degradation of type II collagen in a dose-dependent manner. 60 µM NF157 nearly completely rescues type II collagen from degradation induced by TNF-α (10 ng/mL).
NF157 (30 and 60 µM; 24 hours) almost fully restores nuclear translocation of p65 triggered by TNF-α (10 ng/mL) and significantly reduces the luciferase activity of NF-κB.
Western Blot Analysis
| Cell Line: | SW1353 cells |
| Concentration: | 30 and 60 µM |
| Incubation Time: | 24 hours |
| Result: | Ameliorated TNF-α-induced degradation of type II collagen. |
Western Blot Analysis
| Cell Line: | SW1353 cells |
| Concentration: | 30 and 60 µM |
| Incubation Time: | 24 hours |
| Result: | Reduced TNF-α-induced activation of NF-κB. |
