3,3''-((2-Chlorophenyl)methylene)bis(4-hydroxy-2H-chromen-2-one)

3,3'-((2-Chlorophenyl)methylene)bis(4-hydroxy-2H-chromen-2-one) is a non-nucleotide inhibitor of ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1; K_i = 50 μM).^1,2 It also inhibits urease (IC₅₀ = 84.53 μM for the Jack bean enzyme).³

1. Choudhary, M.I., Fatima, N., Khan, K.M., et al. New biscoumarin derivatives-cytotoxicity and enzyme inhibitory activities Bioorg. Med. Chem. 14(23),8066-8072(2006).
2. Onyedibe, K.I., Wang, M., and Sintim, H.O. ENPP1, an old enzyme with new functions, and small molecule inhibitors - A STING in the tale of ENPP1 Molecules 24(22),E4192(2019).
3. Khan, K.M., Iqbal, S., Lodhi, M.A., et al. Biscoumarin: New class of urease inhibitors; economical synthesis and activity Bioorg. Med. Chem. 12(8),1963-1968(2004).