These PPARα Reporter Assay Systems utilize non-human mammalian cells engineered to express human PPARA, commonly referred to as PPARα. Peroxisome proliferator-activated receptor alpha (PPARα), also known as NR1C1, is a nuclear receptor protein encoded by the PPARA gene. PPAR ligands, whose names derived from their effect on inducing an increase in the size and number of peroxisomes, include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. Multiple alternatively spliced transcript variants have been described for PPARα gene, although the full-length nature of only two has been determined. PPAR Reporter Cells are prepared using INDIGO’s proprietary CryoMite™ process. This cryo-preservation method yields high cell viability post-thaw, and provides the convenience of immediately dispensing healthy, division-competent reporter cells into assay plates. There is no need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments.[INDIGO Catalog Nos. IB00111-32, IB00111, IB00112]WARNING This product is not for human or veterinary use.
