Cayman’s Ammonia Colorimetric Assay Kit provides a colorimetric method for measuring ammonia in plasma, serum, and urine. In the assay, ammonia is converted into NADH in a series of enzymatic reactions. NADH is then oxidized, resulting in the reduction of the tetrazolium salt substrate WST-8 to a highly colored formazan dye, which absorbs at 450 nm. The amount of formazan is proportional to the amount of ammonia in the sample. This assay has a range of 0-60 μM and a lower limit of detection (LLOD) of 1.3 μM.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
