DIG DNA Labeling and Detection Kit

DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques:

• Southern blots
• Northern blots
• Dot blots
• Colony and plaque screening

DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light blue precipitate.

This product is designed as a safer chemical.?The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.