4-(Trifluoromethyl)umbelliferyl Phosphate Disodium Salt is experimentally used as a fluorogenic substrate for alkaline phosphatases. It is also a disodium salt form of 4-(Trifluoromethyl)umbelliferyl Phosphate which can be used in biological study of identification of novel fragment compounds targeted against pY pocket of v-Src SH2 by computational and NMR screening and thermodynamic evaluation.