S-Phenylsulfonylcysteine

S-Nitrosylation reactions regulate protein function and mediate nitrosative stress. S-Phenylsulfonylcysteine is a reagent for blocking thiol (-SH) groups on proteins and peptides at room temperature. This is the first step of the thiosulfonate switch technique, in which thiosulfonates are fluorescently tagged with a probe bearing a reactive thiol, such as rhodamine-SH.

S-Phenylsulfonylcysteine is a reagent for blocking thiol (-SH) groups on proteins and peptides[1].

[1] Reeves BD, et al. Conversion of S-phenylsulfonylcysteine residues to mixed disulfides at pH 4.0: utility in protein thiol blocking and in protein-S-nitrosothiol detection. Org Biomol Chem. 2014 Oct 28;12(40):7942-56. DOI:10.1039/c4ob00995a