MENSACARCIN
MENSACARCIN 性质
| 沸点 | 656.8±55.0 °C(Predicted) |
|---|---|
| 密度 | 1.53±0.1 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | DMSO：可溶，甲醇：可溶 |
| 形态 | 固体 |
| 酸度系数(pKa) | 11.50±0.70(Predicted) |
| 颜色 | 白色至类白色 |
MENSACARCIN 用途与合成方法
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Traditional Cytotoxic Agents
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Mensacarcin (0-100 μM; 24 hours) exhibits general cytostatic but type-specific cytotoxic effects for melanoma cells.
Mensacarcin (2-50 μM; 15 hours) induces rapid apoptotic cell death in melanoma cells.
Mensacarcin exhibits potent cytostatic properties (mean of 50% growth inhibition=0.2 μM) in almost all cell lines of the National Cancer Institute (NCI)-60 cell line screen and relatively selective cytotoxicity against melanoma cells. Mensacarcin is a highly oxygenated polyketide that was first isolated from soil-dwelling
Streptomyces
bacteria. Mensacarcin impairs mitochondrial function in melanoma cells.
Cell Viability Assay
| Cell Line: | SK-Mel-28 and SK-Mel-5 melanoma cells, HCT-116 colon cancer cells |
| Concentration: | 0.01, 0.1, 1, 10, 100 μM |
| Incubation Time: | 24 hours |
| Result: | Induced concentration- and time-dependent cell death in the two tested melanoma cell lines. HCT-116 colon carcinoma cells were strongly inhibited. |
Western Blot Analysis
| Cell Line: | SK-Mel-28, SK-Mel-5 cells |
| Concentration: | 2, 10, 50 μM |
| Incubation Time: | 15 hours |
| Result: | Induced the formation of 89-kDa PARP-1 fragments as well as caspase-3 activation in SK-Mel-28 and SK-Mel-5 beginning between 6 and 15 h after exposure. |
