Cayman’s Caspase-3/7 Cell-Based Activity Assay Kit provides a convenient method of detecting caspase-3 and caspase-7 activity in cell lysates. Activity is monitored using a caspase-3/7 specific substrate, N-Ac-DEVD-AFC. Cleavage by the active enzyme(s) generates a highly fluorescent product that can be measured using excitation and emission wavelengths of 400 and 505 nm, respectively.Active caspase-3 is included in the kit as a positive control and staurosporine is included to induce apoptosis. The caspase-3/7 inhibitor, N-Ac-DEVD-CHO, is included as a control to confirm the specificity of the fluorescence signal to the activity of the enzymes.Needed but not supplied: Please download the kit booklet to verify if UltraPure Water (Milli-Q or equivalent) or any other components are needed for this assay.WARNING This product is not for human or veterinary use.
