Fluorinated VHL Spy Molecule 4 can be used for the discovery of new von Hippel-Lindau (VHL) binders. Based on VH032 (cat#
901490), this reporter was designed in the lab of
Alessio Ciulli to bind the hydroxyproline (Hyp) recognition site of VHL. When used in competition ligand-observed 19F NMR screening experiments, its displacement indicates binding by another molecule. Additional Fluorinated VHL Spy Molecules are available as listings
909416,
909432, and
909440.
The E3 ligase VHL is of growing interest for
targeted protein degradation, and these spy molecules will facilitate the identification of novel VHL ligands and VHL-based degraders.
