Monoclonal anti-neurofilament 160 antibody (diluted 1: 5000) can be used as internal loading controls in immunoblotting. It can also be used in whole-mount immunohistochemistry and western blot. Monoclonal anti-neurofilament 160 antibodies can be used for localization of neurofilaments of molecular weight 160,000 in cultured cells or tissues preparation.
Neurofilaments belong to the intermediate filament family and are expressed mainly in cells or tissues of neuronal origin. It is crucial for proper radial growth of axons.
Neurofilaments play a role in axonal calibre as they help in movement of an impulse down the axon. Their activity depends on phosphorylation of neurofilaments.Mutations of neurofilaments causes Charcot-Marie-Tooth (CMT) disease. Accumulation of neurofilaments has been observed in many human neurological diseases like Alzheimer′s disease, progressive supranuclear palsy, diabetic neuropathy, and giant axonal neuropathy.
