2-[(4-Bromophenyl)methylene]-N-(2,6-dimethylphenyl)-hydrazinecarboxamide

EGA (415687-81-9) is a novel selective inhibitor of endosomal trafficking which blocks cellular entry of lethal toxin and other acid-dependent bacterial toxins and viruses into mammalian cells.1?Inhibits Anthrax lethal toxin-induced RAW264.7 cell death, IC50=1.7 mM.1 Delays lysosomal targeting and degradation of the EGF receptor indicating that it targets host membrane trafficking.1 Does not block endosomal recycling of transferrin, retrograde trafficking of ricin, phagolysosomal trafficking or phagosome permeabilization by Franciscella tularensis, nor does it neutralize acidic organelles.1 Protects mammalian cells from Clostridium difficile CDT, Clostridium perfringens Iota toxin and Clostridium botulinum C2 toxin.2 A useful tool for exploring subcellular trafficking of various bacterial toxins.3

EGA is a selective inhibitor of endosomol trafficking pathways exploited by multiple toxins and viruses.

Gillespie et al. (2013), Selective inhibitor of endosomal trafficking pathways exploited by multiple toxins and viruses; Proc. Natl. Acad. Sci. USA, 110 E4904 Schnell et al. (2016), EGA Protects Mammalian Cells from Clostridium difficile CDT, Clostridium perfringens Iota Toxin and Clostridium botulinum C2 Toxin; Toxins (Basel), 8 101 Dixon et al. (2015), Distinct Roles for CdtA and CdtC during Intoxication by Cytolethal Distending Toxins; PLoS One, 10 e0143977