Butanoic acid, 4,4'-(carbonyldiimino)bis-, 1,1'-bis(2,5-dioxo-1-pyrrolidinyl) ester

The ureido-4,4′-dibutyric acid bis(hydroxysuccinimide) ester, also known as DSBU or BuUrBU, is the longest version of MS-cleavable urea-based lysine-lysine reactive homobifunctional cross-linkers, featuring a C₄-arm. At neutral or slightly basic pH, it irreversibly crosslinks the neighbouring lysine groups. The presence of the symmetrical urea moiety which is prone to collision-induced dissociation allows for the unambiguous distinguishing of crosslinks in tandem CID-MS experiments.

reaction type: C-C Bond Formation
reagent type: cross-linking reagent