Anti-Dog IgG (whole molecule)-FITC antibody may be used for direct immunofluorescence using dog peripheral blood lymphocytes at a minimum working dilution of 1:32.
Anti-Dog IgG (whole molecule)-FITC antibody has been used in indirect fluorescent antibody test(IFAT)
IgG?is present in large quantities in the human serum and about 10-20% of the plasma proteins.?IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates.?It consists of four sub-classes?i.e?IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ?chains)?and two light chains (κ or λ?chains)?which are linked by inter-chain?disulfide?bonds.?The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one?N-terminal variable domain (VL) and?one?constant domain?(CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding).?The antigen binds to the V regions of the?antibody.
IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments. Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495nm and emits at 521nm.
