The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Recommended starting dilution is 1:1000.
Anti-human SNAI1 mAb, clone PAT2D5AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SNAI1 protein 1-264 amino acids purified from E. coli.
Snail homolog 1 (SNAI1) is involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. SNAI1 binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. The nuclear protein encoded by SNAI1 is structurally identical to the Drosophila snail protein, and is considered as well to be vital for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.Among the diseases associated with SNAI1 are waardenburg syndrome type iid, and inappropriate adh syndrome.
