The MILLIPORE Re-Blot Western Blot Recycling Kit is effective for removal of antibodies from Western blots th at have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates th at precipitate at the reaction site. The Re-Blot Western Blot Recycling Kit should be used only for qualitative purposes until it has been established by comparative blot analysis th at stripping does not quantitatively affect a given antigen. This product is for research use only; not for diagnostic or in vivo use.