C12 NBD Sphingomyelin (d18:1/12:0)

C12 NBD sphingomyelin is a biologically active derivative of sphingomyelin (Item Nos. 22674 | 24345) that is tagged with a fluorescent C12 nitrobenzoxadiazole (C12 NBD) group. C12 NBD sphingomyelin has been used to study the metabolism and transport of sphingomyelins.1,2 It is hydrolyzed by sphingomyelinase to C12 NBD ceramide (Item No. 10007958).1 This product is a mixture of the D-erythro and L-threo isomers of C12 NBD sphingomyelin. As this product is derived from a natural source, there may be variations in the sphingoid backbone. [Matreya, LLC. Catalog No. 1619]WARNING This product is not for human or veterinary use.

[1] A. LOIDL. High-precision fluorescence assay for sphingomyelinase activity of isolated enzymes and cell lysates.[J]. Journal of Lipid Research, 2002, 43 5 1: 815-823. DOI: 10.1016/s0022-2275(20)30124-3
[2] T NAKAGAWA. Preparation of fluorescence-labeled GM1 and sphingomyelin by the reverse hydrolysis reaction of sphingolipid ceramide N-deacylase as substrates for assay of sphingolipid-degrading enzymes and for detection of sphingolipid-binding proteins.[J]. Journal of biochemistry, 1999, 126 3: 604-611. DOI: 10.1093/oxfordjournals.jbchem.a022492