2,7-双(2-羧乙基)-5(6)-羧基荧光素
2,7-双(2-羧乙基)-5(6)-羧基荧光素 性质
储存条件 | -20°C |
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溶解度 | DMF:5mg/mL; DMSO:15mg/mL; DMSO:PBS (pH 7.2) (1:2):0.25 mg/ml;乙醇:5mg/mL |
形态 | 结晶固体 |
颜色 | 黄至橙色 |
BRN | 8375770 |
2,7-双(2-羧乙基)-5(6)-羧基荧光素 用途与合成方法
Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K + /H + ionophor nigericin does not inhibit hydrogenosomal loading with BCECF.The pH-sensitive fluorescent dyes to measure cytosolic pH. 1.Prepare a 2 to 20 mM stock solution of BCECF in DMSO. 2.Prepare a 5-50 µM BCECF dye-loading solution in buffer solutions (HHBS or PBS).3. Add 1000 µL/well (6-well plate),100 µL/well (96-well plate) or 25 µL/well (384-well plate) BCECF dye-loading solution into the cell plate.4. Incubate the dye-loading plate in a cell incubator for 30-60 minutes.5. Wash and replace the dye-loading solution with buffers.6. Run the pH assay by monitoring the fluorescence at E x /E m = 490/535 nm or 430/535 nm for ratio measurements.
2,7-双(2-羧乙基)-5(6)-羧基荧光素 价格(试剂级)
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
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2024-11-08 | HY-101882 | 2,7-双(2-羧乙基)-5(6)-羧基荧光素 | 85138-49-4 | 1mg | 1100 |
2024-11-08 | HY-101882 | 2,7-双(2-羧乙基)-5(6)-羧基荧光素 | 5 mg | 3250 |