The purified IgG4, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunobinding, Western immunobloting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell binding assays. Purified IgG4, λ was used as isotype control in flow cytometry of chronic lymphocytic leukemia and mantle cell lymphoma cells. It was also used for lectin- surface plasmon resonance and IgG subclass labelling by flow cytometry at a concentration of 50 μg/ml.
IgG is the main antibody type found in plasma and extracellular fluid and is expressed on B cell membrane. IgG is further subdivided into four subclass- IgG1, IgG2, IgG3 and IgG4. Human IgG4, λ myeloma protein is used to determine the specificity of ‘cytoplasmic human IgG subclass labelling′ assay in cloned Epstein-Barr virus cell line. This product is useful to probe and label CDR152 and can control T cell activation. Human IgG4 λ antibody can also be used in detection and isolation of human CD4+CD25+CD152+ regulatory T cells. This product has shown specificity for human.
