Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA).
Anti-Human IgG (Fab specific) antibody produced in goat is effective as a second antibody reagent in immunoassay procedures. It may be used:
- as starting material for conjugates using enzymes or fluorescent dyes
- in enzyme linked immunosorbent assay
- as a cross-linking antibody in internalisation of 125I-IgG by streptolysin-O-permeabilised cells
- as capture antibody in enzyme-linked immunosorbent assay (ELISA) for detection and semiquantitation of Fab
- in electrophoresis and immunoelectrophoresis
Anti-Human IgG (Fab specific), F(ab′)2 fragment antibody produced in goat has been used in enzyme linked immunosorbent assay (ELISA) and biosensor methodology.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG.
Immunoglobulin G (IgG) is digested by papain and yields two Fab fragments, each of which can bind antigen, and a single Fc fragment. IgG is split by pepsin splits into an Fc fragment and a single dimeric F(ab)2 that can cross-link as well as bind antigens.
