Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse is suitable for immunohistochemistry at a working dilution of 1:400 using rat brain sections (alcohol-fixed) and microarray analysis. It may be used for immunocytochemical localization of GFAP in human, pig, and rat tissues. It is also suitable for localization of GFAP using immunoblot assays. In indirect immunofluorescent labeling on alcohol-fixed or frozen sections, this antibody stains astrocytes and Bergmann glia cells, gliomas, and other glial cell derived tumors.
The antibody was used as a primary antibody in immunocytochemistry analysis:
- of primary cerebral microvascular EC cultures to study the effect of microglia on the BBB (blood-brain barrier) and its primary constituents
- to study the wound healing effects of matrix metalloproteinase-2 that promote recovery after spinal cord injury
- to study the negative regulation of embryonic neural progenitor cell proliferation by Toll-like receptor 3