Cell-free protein synthesis (CFPS) is an easy-to-use, rapid, and efficient system that is devoid of membrane-bound barriers but contains the native cellular transcriptional and translational machinery and exogenous resources (e.g., amino acids, nucleotides, and a secondary energy substrate) needed for synthesis of the desired proteins.1,2,3,4,5 The CFPS (E. coli) Kit can produce high levels and different molecular weights of recombinant proteins within several hours and can be used in high-throughput functional genomics and proteomics. In addition, it can provide proteins to drive biochemical and structural biology research. The synthesized protein can be detected by SDS-PAGE, Western blot, and fluorescence-detection size exclusion chromatography (FSEC) and can be isolated from the reaction mixture by affinity purification techniques for further structural and/or functional characterization.WARNING This product is not for human or veterinary use.
