H3B-120 has no inhibition of CPS2 activity of CAD (CPS2, aspartyl transcarbamylase, dihydroorotase).
It achieves inhibition by binding to an allosteric pocket situated between the integrating and ATP A domains.
Also H3B-120 (25, 50, 75, 100 μM) inhibits urea production in a dose-dependent manner, although the cellular potency decreases significantly compared with enzymatic assays.
The half-life of H3B-120 is only 40 min.