B022 (0-5 μM; 12 hours; Hepa1 cells) treatment suppresses NIK-induced p52 formation in a dose-dependent manner.
B022 (0-5 μM; 12 hours; Hepa1 cells) treatment for 8 h completely blocks NIK-induced expression of TNF-a, IL-6, iNOS, CCL2, and CXCL5.
Western Blot Analysis
| Cell Line: | Hepa1 cells |
| Concentration: | 0 μM, 0.5 μM, 5 μM |
| Incubation Time: | 12 hours |
| Result: | Suppressed NIK-induced p52 formation in a dose-dependent manner. | td> |
RT-PCR
| Cell Line: | Hepa1 cells |
| Concentration: | 0 μM, 0.5 μM, 5 μM |
| Incubation Time: | 12 hours |
| Result: | Dose-dependently blocked NIK-induced expression of chemokines, cytokines, and iNOS in these cells. Completely blocked NIK-induced expression of TNF-a, IL-6, iNOS, CCL2, and CXCL5. |
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