Prostaglandin F2α-1-glyceryl ester-d5

Prostaglandin F2α-1-glyceryl ester-d5 (PGF2α-1-glyceryl ester-d5) is intended for use as an internal standard for the quantification of PGF2α-1-glyceryl ester by GC- or LC-MS. 2-Arachidonoyl glycerol (2-AG) has been isolated from porcine brain, and has been characterized as the natural endocannabinoid ligand for the central cannabinoid receptor.1,2 Incubation of 2-AG with cyclooxygenase 2 and specific PGH2 isomerases in cell cultures and isolated enzyme preparations results in PG glyceryl ester formation.3 The biosynthesis of PGH, PGD, PGE, PGF, and thromboxane A-2-glyceryl ester compounds have all been documented. The 2-glyceryl ester moiety equilibrates rapidly (within minutes) with the more stable 1-glyceryl ester, producing a 10:90 2:1-glyceryl ester mixture in typical aqueous media. While the stability and metabolism of PGF2α-1-glyceryl ester has been investigated, little is known about its intrinsic biological activity.4WARNING This product is not for human or veterinary use.

[1] TAKAYUKI SUGIURA . 2-Arachidonoylglycerol, a Putative Endogenous Cannabinoid Receptor Ligand, Induces Rapid, Transient Elevation of Intracellular Free Ca2+in Neuroblastoma × Glioma Hybrid NG108-15 Cells[J]. Biochemical and biophysical research communications, 1996, 229 1: Pages 58-64. DOI: 10.1006/bbrc.1996.1757
[2] T SUGIURA. Is the cannabinoid CB1 receptor a 2-arachidonoylglycerol receptor Structural requirements for triggering a Ca2+ transient in NG108-15 cells.[J]. Journal of biochemistry, 1997, 122 4: 890-895. DOI: 10.1093/oxfordjournals.jbchem.a021838
[3] KEVIN R KOZAK. Metabolism of the endocannabinoids, 2-arachidonylglycerol and anandamide, into prostaglandin, thromboxane, and prostacyclin glycerol esters and ethanolamides.[J]. The Journal of Biological Chemistry, 2002, 277 47: 44877-44885. DOI: 10.1074/jbc.m206788200
[4] K. KOZAK. Metabolism of Prostaglandin Glycerol Esters and Prostaglandin Ethanolamides in Vitro and in Vivo *[J]. The Journal of Biological Chemistry, 2001, 1 1: 36993-36998. DOI: 10.1074/jbc.m105854200