The arachidonic acid CYP450 metabolite LC-MS mixture contains CYP450 metabolites derived from arachidonic acid (AA; ). The mixture is supplied in an amber ampule in which the headspace has been purged with argon to prevent lipid oxidation. This product has been designed for direct use in LC-MS applications. The solution may be used as a system suitability standard or tuning standard. After opening, we recommend that the mixture be transferred immediately to a 1 ml glass screw cap vial, to prevent solvent evaporation, and stored at -20°C. The mixture should be discarded after multiple freeze/thaw cycles.
This mixture contains (±)14(15)-DiHET , (±)11(12)-DiHET , (±)8(9)-DiHET , (±)5(6)-DiHET , 19(R)-HETE , 20-HETE , (±)14(15)-EET , (±)11(12)-EET , and (±)8(9)-EET , each of which are involved in either vasoconstriction or vasodilation.
