S49076 effectively inhibits the phosphorylation of MET, AXL and FGFRs in cells, and inhibits their downstream signaling pathways in vitro and in vivo. In cellular models, it inhibits MET and FGFR2-dependent gastric cancer cells, prevents MET-driven migration of lung cancer cells, and inhibits colony formation of liver cancer cells expressing FGFR1/2 and AXL. S49076 inhibits cell viability, motile, three-dimensional colony formation of cancer cells expressing MET, AXL or FGFRs.
