MitoP

MitoP is a phenol product produced by the reaction of H₂O₂ with the ratiometric mass spectrometry probe MitoB . MitoB contains a triphenylphosphonium cation component that drives its accumulation in mitochondria where its arylboronic moiety selectively reacts with H₂O₂ to produce MitoP.^1,2 Quantifying the MitoP/MitoB ratio by LC-MS/MS reflects the mitochondrial matrix H₂O₂ concentration.

1. Cochemé, H.M., Quin, C., McQuaker, S.J., et al. Measurement of H₂O₂ within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix Cell Metab. 13(3),340-350(2011).
2. Cochemé, H.M., Logan, A., Prime, T.A., et al. Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H₂O₂ in living Drosophila Nat. Protoc. 7(5),946-958(2012).