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SBA, Soybean agglutinin
Product Name
SBA, Soybean agglutinin
CAS
MF
MW
0
EINECS
MOL File
Mol File
Chemical Properties
storage temp.
−20°C
form
lyophilized powder
biological source
Glycine max (soybean)
Usage And Synthesis
Uses
General Western Blot Protocol:
Glycoprotein sample size: 500ng
Lectin Concentration: 0.1ug/ml
Load samples at 500 ng of glycoprotein per lane
Run 4-20% Bis-Tris SDS page gel
Transfer gel to a PVDF membrane
Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
Wash membrane 5 x 5 minutes with 25ml RIPA buffer
Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.
SBA, Soybean agglutinin Supplier
Sigma-Aldrich
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021-61415566 800-8193336
Email
orderCN@merckgroup.com
Riedel-de Haen AG
Tel
800 558-9160
Email
SIGMA-RBI
Tel
800 736 3690 (Orders)
Email
Related Product Information
Soybean Flour
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