α-Actinin-4 (ACTN4) is a filamentous actin-crosslinking protein and member of the α-actinin protein family with roles in the regulation of cytoskeletal integrity and cell movement, as well as gene transcription.1,2 It is composed of an N-terminal actin-binding region with two calponin-homology repeats, a rod domain containing four spectrin-like repeats that is essential for anti-parallel dimerization, and a C-terminal calmodulin-like domain.1 ACTN4 is ubiquitously expressed in non-muscle cells and primarily localized to the cytoplasm where it participates in cytoskeletal organization.2 However, ACTN4 can be translocated to the nucleus and induce transcription via interaction with nuclear receptors, including estrogen receptor α, retinoic acid receptors (RARs), and the vitamin D receptor, and acts as a transcriptional co-activator of NF-κB. Mutation of ACTN4 induces podocyte injury caused by cytoskeletal dysregulation and proteotoxicity and has been seen in patients with hereditary focal segmental glomerulosclerosis.3 ACTN4 is overexpressed and associated with tumor metastasis in various cancers.2 Cayman's α-Actinin-4 (N-Term) Rabbit Monoclonal Antibody (Clone RM287) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.WARNING This product is not for human or veterinary use.
