ELISA Analysis: 1:50,000 (against the immunogen). Western Blotting Analysis: A representative lot detected steady level of cellular calpain small subunit during the final stage of human CD34+ erythroid progenitor cells differentiation to reticulocytes (Patterson, T., et al. (2009). J. Biol. Chem. 284(21):14547-1457).Western Blotting Analysis: A representative lot detected upregulated calpain small subunit level in atrophied soleus muscle upon hind limbs immobilization by plaster cast among male rats (Sugiura, T., et al. (2003). J. Physiol. Sci. 56(6):393-399).Western Blotting Analysis: A representative lot detected calpain small subunit in human WI-38 fibroblasts (Zhang, W., et al. (1996). J. Biol. Chem. 271(31):18825-18830).Immunocytochemistry Analysis: A representative lot detected increrased plasma membrane calpain small subunit immunoreactivity following ionomcyin treatment of human large cell lung carcinoma LCLC-103H cells by fluorescent immunocytochemistry (Gil-Parrado, S., et al. (2003). J. Biol. Chem. 278(18):16336-16346).Immunoprecipitation Analysis: Representative lots immunoprecipitated calpain small subunit from human WI-38 fibroblasts (Zhang, W., et al. (1996). J. Biol. Chem. 271(31):18825-18830; Zhang, W., and Mellgren, R.L. (1996). Biochem. Biophys. Res. Commun. 227(3):891-896.).Activity Assay: A representative lot inhibited the proteolytic activity of bovine and canine calpain 2, as well as partially purified human erythrocyte calpain I as a result of increased Km and decreased Vmax toward the substrate casein (Mellgren, R.L., and Lane, R.D. (1988). Biochem. Biophys. Res. Commun. 954(2):154-160).
