Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.
