Calhex 231 treatment significantly decreases the proliferation of cardiac fibroblasts.
Calhex 231 treatment significantly downregulates the CaSR, α-SMA, Col-I/III, MMP2/9 expresses. Calhex231 alleviates high glucose-induced myocardial fibrosis in cardiac fibroblasts.
Calhex 231 could inhibit Itch (atrophin-1 interacting protein 4)-ubiquitin proteasome and TGF-β1/Smads pathways, and then depress the proliferation of cardiac fibroblasts, along with the reduction deposition of collagen , alleviate glucose-induced myocardial fibrosis.
Cell Proliferation Assay
| Cell Line: | Primary neonatal rat cardiac fibroblasts (CFs) |
| Concentration: | 3 μM | td>
| Incubation Time: | 24 hours |
Result: | Significantly decreased the proliferation of cardiac fibroblasts. |
Western Blot Analysis
| Cell Line: | Primary neonatal rat cardiac fibroblasts (CFs) |
| Concentration: | 3 μM |
| Incubation Time: | 48 hours |
| Result: | The expression of CaSR, α-SMA, Col-I/III, MMP2/9 were significantly downregulated. < /td> |
