The Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit provides an economical means of distinguishing between the two acetyl-CoA carboxylase isoforms, and between total acetyl-CoA carboxylase and phosphorylated acetyl-CoA carboxylase. The kit includes enough antibody to perform two western blot experiments per primary antibody.
Background
Acetyl-CoA carboxylase catalyzes the carboxylation of acetyl-CoA to malonyl-CoA. It is the key enzyme in the biosynthesis and oxidation of fatty acids. In rodents, the 265 kDa ACC1 form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 is the main isoform in oxidative tissues. However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs.
References
[1] Castle, J.C. et al. (2009) PLoS One 4, e4369.
[2] Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
[3] Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
[4] Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
[5] Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.
Acetyl-CoA Carboxylase 1 and 2 Antibody Sampler Kit Supplier
