N6-Isopentenyladenine-7-glucuronide can be used as analyte in analytical study and preparation processes for profiling of phytohormones and their major metabolites in rice using binary solid-phase extraction and liquid chromatography triple quadrupole mass spectrometry. It can also be used for biological study. Activation of shoot derived cytokinins production by CLE-RS1/2-HAR1 signaling systemically regulate root nodulation and balance nutritional requirements and energy status in Lotus japonicus.