Lipid PPz-2R1 is an ionizable cationic lipid that has been used in the generation of lipid nanoparticles (LNPs) for the delivery of mRNA in vitro and in vivo.1 LNPs containing lipid PPz-2R1 and encapsulating an mRNA reporter selectively accumulate in lungs over the heart, liver, spleen, and kidneys in mice. LNPs containing lipid PPz-2R1 and encapsulating mRNA encoding phosphatase and tensin homolog (PTEN) increase PTEN protein levels in PTEN-deficient Lewis lung carcinoma (LLC) cells. Lipid PPz-2R1-containing LNPs encapsulating PTEN mRNA reduce tumor growth in an orthotopic murine LLC model when administered alone or in combination with an antibody targeting programmed cell death protein 1 (PD-1).WARNING This product is not for human or veterinary use.
